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KMID : 0370920060230010062
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Yeungnam Univercity Journal of Medicine
2006 Volume.23 No. 1 p.62 ~ p.70
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Effect of Progesterone on COX-2 Expression and Proliferation of Prostate Stromal Cell
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Jung Soo-Ryun
Kim Sung-Han
Choi E-Hwa
Park Ji-Eun
Jeon Eun-Mi
Kang Young-Jin
Lee Kwang-Youn
Choi Hyoung-Chul
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Abstract
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Background: Benign prostatic hyperplasia (BPH) is the most common benign tumor in older men; the etiology of this disease remains poorly understood. Testosterone and dihydrotestosterone (DHT) both act as androgen via a single androgen receptor. Testosterone is converted to DHT by 5alpha-reductase in prostatic stromal cells. Progesterone has been reported to inhibit DHT conversion; howevwe, its effect on prostatic stromal cells remains to be elucidated.
Materials and Methods: In this experiment, we investigated the effect of progesterone on androgen receptor expression induced by DHT. We also tested the effect of progesterone on cyclooxygenase-2 (COX-2) expression, as well as prostate stromal cell proliferation using the cell count kit-8.
Results: Progesterone did not cause an increase of prostate stromal cell proliferation. The mRNA expression of the androgen receptor and COX-2 were not changed by progesterone; the expressions of androgen receptor and COX-2 proteins were decreased by progesterone in prostate stromal cells.
Conclusion: These results suggest that in prostate stromal cells, progesterone decreases androgen receptor protein expression, which results in decrement of COX-2 protein expression. This effect might be mediated by post-transcriptional regulation.
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KEYWORD
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Prostate stromal cell, Progesterone, Cyclooxygenase-2
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