Cytochrome P-450(CYP) enzymes are important in catalyzing the biotransformation on many endogeneous compounds and xenobiotics, including drugs and carcinogens. In the present study, effect of nifedipine a voltage dependent calcium channel blocker
on the
induction of CYPIAI and 2BI was investigated. Change of CYPIAI and 2BI activities were measured by using specific enzyme activities and Western blot with monoclonal antibody 1-7-1, increased in liver microsome of nifedipine-treated spontaneous
hypertensive rat(SHR, 30mg/kg.b.w, twice a day for 3days) but not in kidney microsome. CYP2BI, as quantified by benzyloxyresorufin-O-dealkylase activity and Western blot with monoclonal antibody 2-66-3, markedly increased in liver microsome of
nifedipine-treated SHR but slightly in kidney microsome. The results demonstrate that nifedipine is a potent inducer of CYP2BI in SHR.
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