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KMID : 0376219640010010001
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Chonnam Medical Journal
1964 Volume.1 No. 1 p.1 ~ p.11
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Experimental Studies on the Fate of Foreign Proteins
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Abstract
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Ⅱ. The fate of Foreign Proteins in the Liver and Kidney-blocked Rabbits examined by Electrophoretic Analysis
The following results were obtained by observing the change of serum fraction, serum total protein and ruine total protein levels after injection go Egg-Albumin and Gelatine into rabbits, kidneys and livers of which were blocked due to uranium nltrate and carbon terachloride, respectively.
1. Serum total protein level:
It decreased moderately after foreign trotein injection, recovering gradually after a while, Compared with normal rabbits, however, its period of recovering time was a little bit delayed. So was the period of recovering time of Gelatine injected rabbits compared with Egg-Albumin injected ones
2. Urine Total protein level:
Its highest value was observed 30 minutes after foreign protein injection. After then, it rapidly decreased until 12 hours passed. After that the group of rabbits which had the blocked kidney abowed gradual decrease, while the group of rabbits of the blocked liver maintained the value found at around 12 hours after infection
3. Serum protein fractionation:
The Albumin fractionation of both groups of rabbits showed decrement right after injection of foreign protein but gradually recovered, while, when Gelatine was infected, continuous decreasing was observed until 48 hours passed. when Globulin fractionation were examined, no particular change was observed at aplha-Globulin, slight increment at beta-Globulin, and more or less increasing tendency at the gamma-Globulin, which, however, recovered after a while.
A/G ratio resembled the change of Albumin. That is , when Egg-Albumin was ingjected, the A/G ratio decreased but gradually recovered afterwards, while, when Gelatine was infected, the A/G ratio decreased continuously.
Ⅲ. The Fate of Foreign Proteins in Normal and Liver-and Kidney-blocked rabbits detected by Radioisopope(Ⅰ-^(131))
The following results were obtained by observing the distribution in each organ and excretion in urine of foreign proteins, the Egg-Albumin and Gelatine, which were labeled by radioisotope Ⅰ-^(131), injected into normal rabbits and the ones which had blocked kidney and Liver. And, as a control, a group of rabbits was injected with only radioisotope Ⅰ-^(131).
1. The distribution rate of Ⅰ-^(131) contents in each organ except for urine:
a. The control group.
At the initial stage, blood and kidney showed high rate of Ⅰ-^(131) but after 12 hours it gradually decreased and the isotope became more or less ebenly distributed in every organs.
b. Normal group.
Compared with control group, this group showed a high rate in adrenal gland, bone marrow and liver. The former group showed the highest value 30 minutes after injection in kidney, decreasing gradually after then. shile latter group showed only gradual increase in kidney.
c. Kidney-blocked group.
The blood continuously maintained relatively high rate. Kidney and liver also showed moderatedly high rate.
d. Live-blocked group.
Adrenal gland and bone marrow showed gradually increase, while liver showed lower rate than the one of other groups.
No particular change was observed in other organs.
2. The total sum of cpm calculated from both urine and other organ.
a. Control group:
The highest value was observed 30 minutes after infection, but after then rapidly decreased until 6 hours passed, and then gradual decrease followed it.
Almost all of the isotope excreted 24 hours after infection.
b. Normal group:
Like the control group rapid decrease was ween for first 3 hours, but after then it became gradual.
c. Kidney-blocked group:
Only gradual decrease was shown without wpecial change from initial stage.
d. Liver-blocked group:
Relatibely rapid decrease was observed until 12 hours passed but becoming moderate after then.
3. Excretion in Urine:
It is shown in percentages calculated from the total sum of the urine and all of the organs examined.
Highest values were observed 3 hours after injectionin control group, 6 hours in normal group, 12-24 hours in kidney-blocked group and 24 hours in liver-blocked group. Gradual decrease was observed after each of these periods of time.
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