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KMID : 0376219700070030335
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Chonnam Medical Journal
1970 Volume.7 No. 3 p.335 ~ p.351
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Colicin Production of Escherhichia coli Isolated and the Sensitivity of Enteropathogenic Bacteria to the Colicins, and the correlationship of Colicinnogeny and R Factors
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Abstract
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A total of 365 strains of Escherichia coli were isolated from the feces of as many persons of healthy persons and patients living in Chonnam province in 1969, and were studied´ on their production of colicin applying ultraviolet-induced - method and the sensitivity of enter pathogenic organisms such as Salmonella, Shigella, and Vibrio cholerae to the colicins they produced. Furthermore, the correlation between the colicinogeny and R factor of Escherichia coli was investigated. The results obtained were as follows:
Of 365 strains of the isolates, 41 strains (11.2%) produced colicins, being typed by their ability to produce colicins. The strains producing colicin B was most frequently identified at the rate of 48.7% and the strains producing colicin V, E2, and K were isolated with the frequency of 9.7% or 7.3%. The strains producing "unclassified colicins" which inhibited one or more indicator strains but which could not be classified-by the available scheme were encountered, with considerably higher frequency of 26.8%. However, no strain producing colicin El and I which are the most common colicin identified in U.S. A. as well as. Belgium. was found.
The enteropathogenic bacteria were inhibited by various types of, the colicins produced by Escherichia coli isolated 90% of Shigella, 48% of Salmonellae and 34.4%, of Vibrio cholerae were inhibited by the colicins. The wild strains of enteropathogenic bacteria were more sensitive to the colicins than the, laboratory strains.
Noncolicinogenic strains (68.2%) were more frequently sensitive to the colicins than colicinogenic strains (48.7%)
The antibiotic resistant rate of colicinogenic strains (44%) was remarkably higher than that of noncolicinogenic strains (14.6%), and colicinogenic strains were more than noncolicinogenic strains in number of different pattern of antibiotic resistances.
The isolation frequency of R factor in colicinogenic strains (24.4%) was higher than in noncolicinogenic strains (9.8%), suggesting colicinogenic factor is related to the transfer of R factor.
Among 41 strains of colicinogenic strains, the frequency of colicin production of strains carrying R factor was 24.4%, whereas that of strains without R factor was 75.6%, indicating that R factor seems to suppress colicinogenic factor.
The possible important in vitro and in vivo activities of Escherichia coli and interactions between eposomes were briefly discussed.
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