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KMID : 0376219750120020405
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Chonnam Medical Journal
1975 Volume.12 No. 2 p.405 ~ p.423
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Morphological Studies on Absorption of Tracer Substances by Canal Epithelium of Rat Epididymis
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Abstract
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Regional difference of the epididymal canal epithelium of the rat in India ink absorption was studied with light microscope at various time intervals of er administration of India-ink into the canal lumen in the portions of head, body, and tail of the epididymis. Ferritin absorption by the epithelium in the tail portion of the rat epididymis was also studied by electron microscope and amounts of ferritin in absorbed by the principal and vacuolated cells were compared and absorptive processes of ferritin in these cells was examined by varing the time intervals of per ferritin administration.
Results obtained were as follows;
1. In the head portion, India-ink was absorbed by all the epithelial cells fro one hour after administration of India-ink, and gradually increased as the ti e proceeded following India-ink administration, but never reached to infranucle r cytoplasm up to twenty four hours. In the distal segment of the body add tail, India-ink absorbed mainly by the special cells in which India-ink granules began to appear in the cytoplasm at one hour and increased gradually as the tithe progressed following India-ink administration and filled the whole cytoplasm of these cells whereas principal cells contained very little amount of India-ink at any time interval of the experiment.
2. Ferritin was present in the epithelial cells of the epididymal canal in the tail portion of the rat as early as at ten minutes and gradually increased up to on hour but markedly increased at three to twenty four hours after ferric administration. Ferritin absorption by the vacuolated cells was much more promine t than that of the principal cells from the early stage of the absorption. In th vacuolated cells, ferritin was present in small vesicles, vacuoles, and multive sieular bodies up to one hour and increased greatly in these vacuolar structures as the time progressed following ferritin administration although it s sparse in the large mucoid granules of the deeper cytoplasmic region up to three hours. In the later stage, ferritin was densely packed within all the large vacuoles which, in turn, filled the vacuolated cells. The absorption f ferritin by principal cells was also active from thirty minutes to three hour, General picture of the ferritin absorption was more or less similar to that of the vacuolated cells but ferritin containing vacuoles were much less in number and smaller in size, and was found mainly in the supranuclear cytoplasm in the later stage(fifteen hours).
From these results, it is concluded that the absorptive function of the canal epithelium is different from the one portion to the another, and the vacuolated cels have marked absorptive function suggesting that these are absorptive cells rather than secretory cells.
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