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KMID : 0376219760130010069
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Chonnam Medical Journal
1976 Volume.13 No. 1 p.69 ~ p.75
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Preferential Incorporation of Nicotinic Acid into Nicotinamide Adenine Dinucleotide of Rabbit Erythrocytes as Compared with Nicotinamide
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Abstract
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In view of the report by Jaffe and Gordon that thirty-fold greater incorporation of nicotinamide into nicotinamide adenine dinucleotide (NAD) than of nicotinic acid occurred in rabbit erythrocytes which is in marked contrast to the findings with human erythrocytes the present study was carried out to deterrrsine the efficacy of nicotinic acid and nicotinamide as a precursor for NAD biosynthesis in rabbit erythrocytes.
Washed erythrocyte froom norma1 rabbits were incubated at 37°C for various times, with glucose, glutamine, phosphate buffer and nicotinic acid-7-14C or nicotinamide-7-14C The uptake of both precursors by erythrocytes and their incorporations into NAD and pyridine nucleotide intermediates of NAD biosynthesis such as deamido-NAD and nicotinamide ribonucleotide were determined by chromatographic methods.
In marked contrast to the findings of Jaffe and Gordon, six-fold greater incorporation into NAD with three-fold greater incorporation into deamido-NAD and nicotinamide ribonucleotide of nicotinic acid than of nicotinamide was observed. The uptake of nicotinic acid was also slightly greater than of nicotinamide.
Since- three-fold greater net synthesis of NAD from nicotinic acid than from equimolar nicotinamide occurred during 4 hours incubation, it might be concluded that as with human erythrocytes, rabbit erythrocytes also utilizes nicotinic acid better than nicotinamide as a precursor for NAD, biosynthesis.
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