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KMID : 0377619640070040439
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Korean Jungang Medical Journal
1964 Volume.7 No. 4 p.439 ~ p.453
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Studies on the Lysogeny in Staphylococci
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Abstract
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The propagating strains (PS) of phages, 29, 52, 52A/79, 80, 81 and 82, were studied on the lysogenization with phages 80, 81 and 82. Strains belonging to phage types 80, 80/81 and 52/52A/80/81, which were isolated in this laboratory from patients suffering from staphylococcal infections, were also subjected for this study.
The main purposes of this study are to know the lysogenizing abilities of phages in epidemic strains under ultraviolet-irradiated and non-irradiated conditions, and the changes in phage patterns following lysogenization The change in antibiotic resistance following lysogenization and the reversion in phage sensitivity of lysogenized strains were also studied. The following results were obtained.
1. PS 29, 52, and 52A/79 were not lysed by phages 80, 81 and 82, and not lysogenized by these phages.
2. All PS were not lysogenized by phage 80. However, ultraviolet-irradiated PS was lysogenized by phage 80, and non-typable strains were derived,
3. PS 80 and 81 were lysogenized by phage 81 belonging to serological A group, and lysogenized strains showed phage patterns of 52/52A/79/80 and 80.
4. PS 80, 80 and 82 were lysogenized by serological group A phage 82 and non-typable strains were obtained.
5. Irradiation of PS with ultraviolet did not result in the increase in the frequency and tendency of lysogenization with phages except PS 80.
6. Some of isolated strains belonging to 80/81-52/52A/80/81 complex were lysogenized by phages 80 and 81. Lysogenized strains became immune to phages serologically belonging to the same group with the lysogenizing phages.
7. Ultraviolet irradiated isolated strains were more frequently lysogenized than non-irradiated ones by phages 80, 81 and 82.
8. Phage patterns of lysogenized strains were stable and did not changed by repeated subcultures.
9. The abilities to produce coagulase and pigment and to ferment mannitol. were the same as those of the parent strains.
10. Penicillin resisiance of PS of lysogenizing phages was transduced into lysogenized strains derived from penicillin-sensitive PS, Sensitivity of penicillin and streptomycin in PS of lysogenizing phages was transduced into the strains derived by lysogenization of strains resistant to penicillin and streptomycin.
11. Lysogenized non-typable strains have a tendency to become sensitive, by treatment with acrif ravine, to phages for which parent strains were sensitive, but lysogenized typable strains did not show any tendency of reverting sensitivity.
12. Host ranges of temperate phages in typable and nontypable lysogenized strains were almost the same as those of phages used for lysogenization.
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