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KMID : 0377619710210040309
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Korean Jungang Medical Journal
1971 Volume.21 No. 4 p.309 ~ p.318
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Effect of Higher Concentration of Sodium Chloride on the Klebsiella Phage-Host Cell System
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Abstract
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The author investigated the effect of higher concentration of NaCl on Klebsiella phage-host cell system and obtained the following results.
The phage activity was relatively stable in higher concentration of NaCl. 1.0% of NaCl did not give any special influence to the growth of host bacteria and, when the concentration of NaCl increase above than 1.0%, the bacterial. growth was gradually inhibited. The highest concentration of NaCl in which host bacteria can multiply was about 4.0%.
The higher the concentration of NaCl in media was, the lower became the grade of phage growth, even though the phage is already adsorbed for 7~10 minutes under normal condition. If the host bacteria were sub cultured, until 5th. generation, in the media contained NaCl of 4.0%, no any special change was found in the efficiency of plating of phage.
The adsorbing rate of phage to host cells became gradually lower by increasing of NaCl concentration and, if higher concentration of 1NTaC1 is introduced into the phage-host cell system after manipulating of phage adsorption for 4 minutes under normal condition, the number of unabsorbed phages increased somewhat according to progressing of time.
The phage burst was considerably delayed and phage yield became also lower even though higher concentration of NaCl is introduced at the late period of minimum latent period of phage.
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