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KMID : 0377619730240040359
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Korean Jungang Medical Journal
1973 Volume.24 No. 4 p.359 ~ p.370
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Active Center of Transport ATPase
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KOH IL-SUP
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Abstract
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The aim of present study is to summarize the evidence that justifies the active center of transport ATPase. 1) The ionic pump required the energy comes from the hydrolysis of ATP and the pump in the intact cell was inhibited by low concentrations of ouabain. Experiments on ghosts showed that these substances in similar low concentrations inhibited just that part of the ATPase activity that depended on the presence of sodium plus potassium ions. 2) The loss of potassium from the NaF poisoned rabbit red cells was abolished by repeatedly washing with isotonic saline solutions´ which indicating that this enzyme and substrate combine reversibly to form a complex. 3) The recovery of cell membrane poisoned by Pb++ occurred in the 303 minutes. The results suggest that as soon as administered Pb++ will first contact the cell membrane to be forming a S>Pb complex and the cell membrane became a leaky membrane, and thereafter the recovery of the leaky cell membrane was reconstituted by to be .forming of a s-Pb-s complex which could not be induced the leakage of potassium from the red cells. 4) In a parallel study, it showed that the loss of potassium from the red cells which were poisoned by Pb++ was not occurred by the addition by cysteine but occurred by EDTA. On the basis of these experimentations it was suggested that the SH groups do act as a active center of transport ATPase which is intimately. involved in the active transport of electrolytes across biologic membranes.
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