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KMID : 0378019690120030051
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New Medical Journal
1969 Volume.12 No. 3 p.51 ~ p.57
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The Enhancement of Plaque Formation in Tissue Culture (VERO-cell) by Arboviruses
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Abstract
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A number of. arboviruses, such as group A (SF, Chik, WEE) and group B (D-2, RSSE, WN) multiply in mammalian cells but some fail to proceduce plaques -in them when grown under an agar overlay.
The growth. of the plaque by :SF, Chik, WM, Dengue type 2, RSSE, WN viruses were sonie inhibited :in 5% &im milk in agar but were ,favourably .;influenced by addition -of DEAF-dextran in optimal .concentration, 50 to :100: .14g/nil to . agar.. Addition of rprotamine sulfate in optimal concentration, 50 to 100 pg/ml, also increased the plaque formation by.SF,, ck, WEE and WN viruses tested.
The addition of these chemical reagents in optimal concentrations do not inhibit plaque sizes and number, those plaques .by some viruses bear ;polymorphic .ppearances.
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