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KMID : 0378019710140050133
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New Medical Journal
1971 Volume.14 No. 5 p.133 ~ p.145
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An Autoradiographic Study of Effects of 5-Fluorouracil on Protein Synthesis of Pancreas of Mice Using Phenylalanibe-C^(14)
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Abstract
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Young adult male mice of Balb C/Strain were used throughout the experiment. In a previous experiment, it was found that after 3 intramuscular injections of 25mg/kg body weight of 5-fluorouracil the body weights of the Balb/C Strain mice dropped perciptiously during the first 5 days which was followed by a gradual recovery towards a near normal level in 2 weeks. Therefore, the same regimen was used in the present study.
Thirty six mice were paired according to weight and one of the pairs was injected with 3 daily injections of 25mg/kg of 5-fluorouracil. Following the last injection, 3 pairs each were sacrificed on days 1, 3, 5, 7, 10 and 14.
Sixty minutes prior to sacrifice 5μc/gm body weight of phenylalanine-C14 (specific activity 250mc/mM)
were injected. Piece of pancreas from each animal were fixed in paraformaldehyde in cacodylate buffer
and double embedded in parlodionparaffin and sectioned at 4μ and stained with toluidine blue O.
The present study is pertinent to this question as it illustrates the degree of suppressive effects on
protein synthesis of one of the most widely used nucleotide analogues in cancer chemotherapy. The results permit the conclusion.
1. It is shown that, at the level of 100mg/kg body weight, most animals die by day 8, whereas lOmg/ kg produced significant initial decrease of body weight during the first 5 days followed by a gradual recovery thereafter. Since nearly complete recovery was observed with 25 mg/kg, this dose was employed in the major experiments.
2. Following administration of 5-fluorouracil to mice for 5 days the pancreas becomes large and pale. Microscopically,_ many of the acinar cells are enlarged, lack the normal basophilia and =show a marr ed
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