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KMID : 0378019800230120087
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New Medical Journal
1980 Volume.23 No. 12 p.87 ~ p.98
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Effect of Cycloheximide on the Liver of Fasting Mice
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Abstract
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It is well known that cycloheximide inhibits the protein synthesis in the mammalian cells. Therefore, the cycloheximide would induce the morphological changes on the liver of the experimental animals.
The authors have demonstrated the effect of cycloheximide on the liver of mature albino mice morphologically, observing the cellular changes and accumulation of fat in the hepatic parenchymal cells of the fasting albino mice treated with cycloheximide.
A total of 33 healthy albino mice were divided into two groups; the control and the cycloheximide treatment groups. The animals of the experimental groups were deprived of food, but drinking water was available ad libitum from 12 hours prior to administration of cycloheximide until to sacrifice. The animals of cycloheximide treatment group were given 4 mg per kg of body weight of cycloheximide ciluted in physiological saline solution by intraperitoneal injection.
The animals of the control group were given only physiological saline solution. The animals of the experimental groups were sacrificed at intervals of 6, 12 and 24 hours after administration of cycloheximide, respectively.
Histological preparations were prepared by way of hematoxylin and eosinstain for cellular changes of the liver and oil red 0 stain for fat deposits in the hepatic parenchymal cells of fasting albino mice.
It is showed cellular swelling accompanied by the vesicular formation and also revealed fat deposits in the hepatic parenchymal cells of periportal zone of the liver in the cycloheximide treatment mice.
Consequently it is suggested that cycloheximide would induce cellular swelling associated with vesicular degeneration and fatty degeneration in the hepatic parenchymal cells of the fasting mice.
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