|
KMID : 0378019840270120107
|
|
New Medical Journal
1984 Volume.27 No. 12 p.107 ~ p.112
|
|
|
Effects of Vitamin A, Vitamin E, β-Naphthoflavone on Lipid Peroxidation Induced 2-Acetylaminofluorence in Rat Liver Microsomes
|
|
KIMM, Seung-Won
JUHNN, Yong-Sung/KWON, Tae-Hee/PARK, Chang-Sun
|
|
|
Abstract
|
|
|
|
To elucidate the relationship between lipid peroxidation and cytochrome P450 in rat lipid peroxidation was induced by 2-acetylaminofluorene (AAF), followed by determination of cytochrome P450, cytochrome b5, and malondialdehyde. Administration of AAF to the rat 2 hours prior to sacrifice of them increased the content of the microsomal cytochrome P450, in liver significantly (p<0. 001); treatment with p-naphthoflavone (p-NF), vitamin A and vitamin E inhibited the effects observed by AAF (p<0.05). When the rats were injected with AAF, the content of microsomal cytochrome bs in the liver decreased slightly. But it decreased markedly in rats pretreated with p-NF, vitamin A, or vitamin E prior to AAF´ injection (p<0.001). The formation of malondialdehyde was increased by AAF and p-NF (p<0. 01), and the AAF-induced lipid peroxidation was inhibited by pretreatment with high dose of vitamin A (2, 000I.U.) or vitamin E. Lipid peroxidation and cytochrome P450 were observed to be correlated closely, suggesting that lipid peroxidation might be mediated by cytochrome P450 in hepatic microsomes.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|