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KMID : 0378119950220020553
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Chungnam Medical Journal
1995 Volume.22 No. 2 p.553 ~ p.560
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Diagnosis of Hepatitis C using Two Step Polymerase Chain Reaction
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Abstract
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Hepatitis C infection is known to be a very serious disease and high risk of transmission by nonparenteral route. But immunoassay method was less sensitive and specific than that of PCR method. The extraction of RNA is largely important to
amplification
of hepatitis C virus.
This study was performed to evaluate the new reagent, Tri-reagent, which was used to extract RNA compared to the conventional method : heat and phenol-chloroform extraction method.
@ES The results were as follows
@EN 1. Mean concentration of extracted RNA by Tri-reagent method was 6135.3 mg/ml.
2. Mean concentration of extracted RNA by phenol chloroform method was 419.0 mg/ml.
3. Mean concentration of extracted RNA by heat method was 691.7 mg/ml.
4. PCR products by Tri-regent were the most distinctive than those by other two methods.
The mean concentration of extracted RNA by Tri-reagent method was much higher than that of RNA by phenol - chloroform and heat method.
In conclusion, the usage of Tri-reagent was rapid and succesful to extract of RNA compared to the conventional extraction methods.
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KEYWORD
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