KMID : 0379120120400040224
|
|
Korean Journal of Mycology 2012 Volume.40 No. 4 p.224 ~ p.228
|
|
Analysis of Protein Domain for Interaction between ¥á-COP and ¥å-COP in Aspergillus nidulans
|
|
Song Eun-Jung
Kim Ki-Hyun Lee Hwan-Hee Park Jeong-Seok Kang Eun-Hye Park Hee-Moon
|
|
Abstract
|
|
|
In order to screen interactor(s) of the Aspergillus nidulans ¥á-COP of COPI vesicle, we performed the yeast two hybrid screening by using the gene for A. nidulans ¥á-COP as a bait and identified ¥å-COP of the COPI vesicle as an interacting protein. The A. nidualns gene for the ¥å-COP was designated aneA+ (A. nidulans epsilone-COP), which encoded 296 amino acid residues with high level of identity with orthologs from other fungi. Domain analyses with yeast two-hybrid system suggested that the interaction between ¥á-COP and ¥å-COP relied on the C-terminus of both proteins, and that the N-terminal WD domian of ¥á-COP and the TPR region of ¥å-COP were not essential but required for the enhancement of the interaction. These results indicate that the interaction mode between ¥á-COP and ¥å-COP of COPI vesicle is evolutionarily well conserved in eukaryotes.
|
|
KEYWORD
|
|
Aspergillus nidulans, ¥á-COP, ¥å-COP, Yeast two-hybrid assay
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|