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KMID : 0380019930080050504
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Korean Journal of Biotechnology and Bioengineering
1993 Volume.8 No. 5 p.504 ~ p.507
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Effects of Extracellular Proteins on the Recovery of Embryogenic Potential inn Long-term Cultures of Daucus carota
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Abstract
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A declining tendency in embryogenic capability was seen during 6 months culture period during which embryo production decreased from 1000 embryos/ml to 500 embryos/ml. The presence of extracellular factors extracted from newly established embryo cultures restored the embryogenic capability and even enhanced the embryo production up to 5 times (2500 embryos/ml) for old carrot suspension cultures compared with that of control cultures. The stimulating effect on the embryo production indicates that the enhancing effect comes from extracellular compounds that are probably protein molecules.
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