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KMID : 0380019940090030306
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 3 p.306 ~ p.311
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Optimization of the Optical Resolution of Racemic α-Methylbenzylamine Catalyzed by Enzymatic Reaction in Organic Media
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Abstract
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Optical resolution of racemic a-methylbenzylamine was carried out by using Bacillus licheniformis protease in organic media. Enantioselective amidation of racemic amine with an ester as an acyl donor was successfully employed to resolve the racemate. To enhance reaction rate and enantioselectivity, p11 -adjustment by lyophilization of enzyme dissolved in buffe;colyophilization with salts or lyoprotectants, selection of solvents and molecular design of esters were investigated. The optimizai inn of the resolution reaction achieved about 30 fold increase in initial reaction rate and about 1" G i~ crease in enantioselectivity, respectively.
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