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KMID : 0380019950100020204
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 2 p.204 ~ p.212
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Preparation and Characterization of Pe Liposomes Containig Antibody
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Abstract
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A target-sensitive liposome was prepared by using a dioleoyl -phosphatidylethanolamine (DOPE) and a palmitic acid coupled antibody (p-IgG). For the preparation of stable PE-liposomes the key factors such as antibody modification method with palmitic acid, molar ratio of p-IgG to lipid and the amount of various additives, were examined. The optimum molar ratio of p-IgG to lipid was found to be 2.5 x 10-` and the final concentration of deoxycholate for the stable liposome formation was about 0.09%. Two kinds of target-sensitive liposomes, containing polyclonal anti-SRBC(Sheep Red Blood Cell)-antibody and monoclonal anti-d-HCG(Human Chorionic Gonadotropin) -antibody, were successfully prepared. The destabilization of liposomes was examined by measuring the release of calcein entrapped the liposome vesicles. Calcein was released only when the liposomes were contacted with the specificific target cells. The calcein release with non-specific target cells was negligible. From this result it is clear that p-IgG is indispensible for the maintenance of stable PE-liposome and the calcein release is mainly due to the specific interactions between the liposomes containing antibody and the target cells containing antigen.
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KEYWORD
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