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A fungal strain, designated Aspergillus sp. JS-4;was isolated and shown to produce an extracellular polysaccharide used as a bioflocculant. The optimal medium composition for the production of flocculant with Aspergillus sp. JS-42 was glucose 3.0%, yeast extract 0.2%, (NH,)2SO, 0.1%, and CaC12 0.05% in distilled water. The optimum culture temperature and optimum culture pH for the production of the flocculant were 251C and pH 7.0, respectively. The highest production of flocculant was observed after 90 hours of cultivation at the optimal condition. The flocculant could efficiently flocculated the tested solids suspended in aqueous solution and was stable at high temperature(1000) and to pH range of from 2 to 10. The flocculant seems to be a kind of high viscous polysaccharide.
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