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KMID : 0380019990140030259
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Korean Journal of Biotechnology and Bioengineering
1999 Volume.14 No. 3 p.259 ~ p.263
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Investigation of Hydrolysis Characteristics of Fish Oil by Means of Aspergillus oryzae Lipase Lipolase-100T
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Woo Dong-Jin
Cho Kwi-Joon
Hur Byung-Ki
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Abstract
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Fish oil was hydrolyzed with Aspergillus oryzae lipase, Lipolase-100T. The hydrolysis characteristics of Lipolsae-100T were investigated. Lipolase-100T showed 1,3-positional specificity which hydrolyzed acyl chains combined on the 1 or 3 position of triglyceride into free fatty acids. Lipolase-100T represented another property that the saturated fatty acids composing the triglyceride were hydrolyzed more easily that the polyunsaturated fatty acids(PUFAs). n-3 PUFAs, such as C16:4, C20:5 and C22:6, were hardly hydrolyzed, so that the concentrations of those in the mixture of glycerides were increased according to hydrolysis time. Especially docosahexaenoic acid(DHA), C22:6 showed the highest increase in the concentration. This result explained that n-3 PUFAs were combined on 2-position of triglyceride. When the hydrolysis of fish oil with Lipolase-100T 0.4 wt% was performed for 120 hr, n-3 PUFAs wt% was increased to 50 wt% in the mixture of glycerides. This result was obtained due to the 1,3-positional specificity of Lipolase-100T and positional specificity of n-3 PUFAs.
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KEYWORD
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Aspergillus oryzae lipase, 1,3-positional specificity, Lipolase-100T polyunsaturated fatty acids(PUFAs), docosahexaenoic acid(DHA)
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