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KMID : 0380020000150050537
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Korean Journal of Biotechnology and Bioengineering
2000 Volume.15 No. 5 p.537 ~ p.539
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Purification and Characterization of Paclitaxel from Plant Cell Cultures of Taxus Chinensis in Large-Scale Process
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김진현:Kim Jin-Hyun
기은숙:Gi Un-Sook/민범찬:Min Bum-Chan/최형균:Choi Hyung-Kyoon/홍승서:Hong Seung-Suh/이현수:Lee Hyun-Soo
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Abstract
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In developing a HPLC purification process, it was hoped that a single chromatographic system would be sufficient to abtain pure paclitaxel in high yield. However, no such system was found, due in part to the complex taxoid profile of crude paclitaxel and to the rigorous nature of the product specification. A two step HPLC purification was adopted using reverse-phase separation on C(sub)18 as a first step, and normal-phase separation on silica as the final polishing step. Impurity profiles were established and maintained for paclitaxel, which identified and quantified each impurity observed in purified paclitaxel from these two steps, all impurities at or above 0.1% were identified. Results provide information for improving the quality control of paclitaxel production.
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KEYWORD
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plant cell, Culture, paclitaxel, impurity, purification, identification
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