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KMID : 0380020030180030170
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 3 p.170 ~ p.173
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Biophoton Emission of MDCK Kidney Cell with ROS(reactive oxygen species)
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Baik Ku-Youn
Cheon Byung-Soo
Lim Jae-Kwan
Lee Seung-Ho
Soh Kwang-Sup
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Abstract
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This study was studies biophoton characteristics of Madin-Darby canine kidney cells under the influence of CsA and each cell type (mock, wt, R55A) by employing a Photomultipliertube. was used for producing reactive oxygen species (ROS) in this measurement. ROS is also generated during oxidative metabolism in living organism. Images from a fluorescence show an increase of photon intensity emitted from the sample on the influence of CsA and each cell type (mock, wt, R55A). It is believed chemiluminescence (CL) occurred by ROS is responsible for the biophoton emission. hence PMT measurement might be considered as a useful tool for studying biochemical characteristics in relation to ROS
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KEYWORD
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Biophoton, MDCk, cell, ROS, PMT
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