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KMID : 0380020050200040285
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 4 p.285 ~ p.290
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Profiling of Metabolites and Proteins from Eschscholtzia californica induced by Yeast Extract
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Cho Hwa-Young
Park Jeong-Jin
Yoon Sung-Yong
Park Jong-Moon
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Abstract
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Benzophenanthridine alkaloids - sanguinarine, chelirubine, macarpine, and chelerythrine are produced from Eschscholtzia californica (Californica Poppy, used as a sedative by Native Americans) and most of them are derived from dihydrosanguinarine. The properties of sanguinarine are the basis of its antimicrobial activity and its use in chemosurgery and skin cancer excision. For overproduction of sanguinarine from E. californica, yeast extract was used as elicitor and the elicited cell’s metabolites were checked. Sanguinarine production was increased intracelluarly about 8 times in the cell and 5 times extracelluarly. We have peformed proteomic analysis of proteins sequentially extracted from E. califormica suspended cells which were cultured with elicitor, an increase of spot intensity was seen at 24 hours following elicitation. These proteins were separated by two-dimensional electrophoresis (2-DE). We found several spots that were expected to be related to benzophenanthridine alkaloids production by comparing the production profiles of metabolites such as sanguinarine. These results demonstrate the use of metabolite analysis as a tool for detecting target proteins related to metabolites production pathway.
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KEYWORD
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Eschscholtzia californica, sanguinarine, two-dimensional electrophoresis
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