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KMID : 0380020060210040255
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.255 ~ p.259
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Cytotoxicity of Hyaluronic Acid Membrane Cross-Linked with Lactide
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Kim Won-Jung
Kwon Ji-Young
Cheong Seong-Ihl
Kim In-Seop
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Abstract
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The biodegradable hyaluronic acid (HA) membranes cross-linked with lactide using the crosslinking agent, 1-ethyl-3- (3-dimethyl aminopropyl) carbodiimide (EDC) were prepared as a potential biocompatible material for tissue engineering. HA membranes having different mechanical properties were synthesised by varying degree of the mole ratio of lactide to HA, EDC concentration, and crosslinking temperature. HA membranes were degradable in water solution and the degradation became slower with the increasing mole ratio of lactide to HA. HA membranes were sterilized using ethylene oxide gas and extracted with cell culture medium for 24 h at 37℃ and 200 rpm. Cytotoxicity of the extract was tested using NIH/3T3 mouse embryo fibroblast as a model cell. Growth inhibition was not observed in the extracts of HA membranes with the mole ratios of lactide to HA, 5 or 10, and 10% EDC concentration, however 11% of growth inhibition was observed in the extract with the mole ratio of 13. Growth inhibition was not observed in the extracts of HA membranes prepared with 5% EDC or 10% EDC and the mole ratio of lactide to HA, 10, however 12% of growth inhibition was observed in the extract with 20% EDC. Cytotoxicity was not observed in the extracts of HA membranes prepared at varying crosslinking temperatures, 15℃, 25℃, and 28℃ with the mole ratio of lactide to HA, 10 and 10% EDC.
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KEYWORD
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Biodegradable polymer, hyaluronic acid, Lactide, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide, EDC, cytotoxicity
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