|
KMID : 0380020060210050384
|
|
Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.384 ~ p.388
|
|
|
The Production of Lincomycin by Repeated Batch Cultures of Immobilized Streptomyces Lincolnensis
|
|
Kim Chang-Joon
Chun Gie-Taek
Chang Yong-Keun
Kim Sung-Bae
|
|
|
Abstract
|
|
|
|
The production stability of high-yielding mutants of Streptomyces lincolnensis immobilized on celite beads was examined in repeated batch cultures. We also explored the feasibility of immobilization of vegetative mycelial cells on pre-wetted celite beads, which is practical method for cell immobilization. Repeated transfer of immobilized cells into fresh medium every 10 days increased productivity of immobilized cells and maximum concentration of lincomycin, 1007 (±256) mg/L, was obtained at the end of the ninth cycle. A 1.4-fold higher productivity was obtained in immobilized-cell culture than that obtained by suspended-cell culture. When pre-wetted beads were inoculated with vegetative mycelia and cultured a slightly higher amount of immobilized cells and lincomycin was obtained more than those obtained by culture of spores immobilized on dry beads. This result indicates that immobilization of mycelial cells on pre-wetted beads was readily available. This technique is simple and no additional facilities are required for cell immobilization.
|
|
|
KEYWORD
|
|
|
Celite beads, immobilized Streptomyces Lincolnensis, Lincomycin, repeated-batch cultures, production stability
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|