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KMID : 0380020060210050389
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.389 ~ p.393
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Production of β-agarase in Batch and Fed-batch Culture by Agarivorans sp. JA-1
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Lee Song-Ae
Kim Jin-Uk
Jung Jong-Geun
Kim In-Hae
Lee Sang-Hyeon
Kim Sang-Jin
Lee Jae-Hwa
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Abstract
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Characteristics of β-agarase production of Agarivorans sp. JA-1 isolated from north-eastern sea of Jeju marine environment was studied. Optimal cell growth was definite that the medium containing agar is 0.2%. The decreasing pattern of viscosity and agar concentration was same and they reached almost zero after 15 hours. Fed-batch culture was studied to improve agarase productivity by Agarivorans sp. JA-1 in marine broth containing 2.0 g/L agar with intermittent addition of 0.8 g agar two times. The hydrolysis products were identified oligosaccharide of degrees of polymerization 6.
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KEYWORD
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Agarivorans sp. JA-1, agar, agarase, oligosaccharide, fed-batch culture
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