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KMID : 0380020070220010062
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 1 p.62 ~ p.65
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Development of L-Threonine Producing Recombinant Escherichia coli using Metabolic Control Analysis
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Choi Jong-ll
Park Young-Hun
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Abstract
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New strain development strategy using kinetic models and metabolic control analysis was investigated. In this study, previously reported mathematical models describing the enzyme kinetics of intracellular threonine synthesis were modified for mutant threonine producer Escherichia coli TF5015. Using the modified models, metabolic control analysis was carried out to identify the rate limiting step by evaluating the flux control coefficient on the overall threonine synthesis flux exerted by individual enzymatic reactions. The result suggested the production of threonine could be enhanced most efficiently by increasing aspartate semialdehyde dehydrogenase (asd) activity of this strain. Amplification of asd gene in recombinant strain TF5015 (pCL--asd) increased the threonine production up to 23%, which is much higher than 14% obtained by amplifying aspartate kinse (thrA), other gene in threonine biosynthesis pathway.
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KEYWORD
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Metabolic control analysis, threonine, Escherichia coli, aspartate semialdehyde dehydrogenase
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