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KMID : 0380020080230010059
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 1 p.59 ~ p.64
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Production of casein phosphopeptides using Streptococcus faecalis var. liquefaciens cell immobilization
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Kweon Dae-Hyuk
Park Ki-Moon
Jin Yong-Su
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Abstract
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Optimum conditions for production of casein phosphopeptides (CPP) from sodium casenate by immobilized cell culture of Streptococcus faecalis var. liquefaciens were investigated. Immobilized cells were made by mixing 60% sodium alginate solution with an equal volume of culture broth at the end of exponential phase and subsequently dropping the mixture into solution. Optimum conditions for CPP production by the immobilized cells were the same as those (, pH 7.0, and 10% substrate concentration) by the crude enzyme solution from the supernatant of culture broth. Optimum loading volume of the immobilized cells into a batch reactor was 30% (w/v). Using a continuous reactor loaded by the immobilized cells under the identified optimal conditions, we were able to produce CPP continuously up to 30 days with a maximum CPP conversion efficiency of 20%.
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KEYWORD
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Casein phosphopeptides (CPP), Streptococcus faecalis var. liquefaciens
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