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KMID : 0380020080230020131
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 2 p.131 ~ p.134
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Production of kaempferol by enzymatic hydrolysis of tea seed extract
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Lim Yun-Young
Kim Eun-Ki
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Abstract
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Tea seed extract, a byproduct of tea processing, contains two kaempferol glycosides, camelliaside A and camelliaside B. Kaempferol was produced by enzymatic hydrolysis of glycosides. Optimum reaction conditions were investigated. was effective, producing kaempferol in 48 hrs. Optimum temperature and pH were and 4, respectively. Ratio of substrate and enzyme affected the yield. Under optimum conditions, 1.6g kaempferol per 1 kg tea seed extract was produced and 80% of kaempferol precipitated. This result shows that kaempferol could be produced mildly and effectively using tea-processing byproduct.
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KEYWORD
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Tea Seed Extract, Kaempferol, Enzymatic Hydrolysis
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