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KMID : 0380020090240010025
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 1 p.25 ~ p.29
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Biosynthesis of ceramide by deletion mutant of Saccharomyces cerevisiae
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Kim Se-Kyung
Noh Yong-Ho
Yun Hyun-Shik
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Abstract
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Ceramide is important not only for the maintenance of the barrier function of the skin but also for the water-binding capacity of the stratum corneum. Though the effectiveness of ceramide is not understood fully, ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries. However, ceramide production from Saccharomyces cerevisiae has not been widely studied and the quantity are very low. Gene deletion in the cell is used frequently to investigate the function of gene and verification research of drug target. Specially, deletion mutant library is useful for a large amount functional analysis of gene. In this study, deletion mutants of genes on the metabolic pathway of ceramide synthesis in S. cerevisiae were grown in a batch culture and the cellular content of ceramide was measured. The ceramide content was highest in ydc1 mutant and 6 mg ceramide/g cell was obtained.
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KEYWORD
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Saccharomyces cerevisiae, deletion mutant, ceramide
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