|
KMID : 0380020090240010070
|
|
Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 1 p.70 ~ p.74
|
|
|
Comparison of cellulolytic enzyme productivities in various semicontinuous culture modes of Trichoderma inhamatum KSJ1
|
|
Li Hong-Xian
Kim Seong-Jun
|
|
|
Abstract
|
|
|
|
For continuous culture of cellulolytic enzymes production to saccharify food wastes, refill concentration of Mandel’s medium for continuous culture was 0.5%, and refill intervals were determined to 12 hours by analysis of COD and total nitrogen concentration after 4-days batch culture in flask level. As a result, amylase and FPase productivities were 3.5 and 1.0 U/L.hr, respectively. In 10 L bioreactor, the batch culture mode was compared with fed-batch, fill-and-draw for continuous production of cellulolytic enzyme. Enzyme productivities were most high at batch culture and followed by fed-batch culture. Amylase and FPase activities were 42.3 and 5.6 U/L.hr at batch culture, and 23.0, 2.8 U/L.hr at fed-batch culture, respectively. As a result, in continuous cultivation of cellulolytic enzymes by T. inhamatum KSJ1, the mode of fed-batch was most effective in 10 L bioreactor.
|
|
|
KEYWORD
|
|
|
Cellulolytic enzyme, productivity, semicontinuous culture, fed-batch, fill and draw, Trichoderma inhamatum KSJ1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|