KMID : 0380020090240060508
|
|
Korean Journal of Biotechnology and Bioengineering 2009 Volume.24 No. 6 p.508 ~ p.514
|
|
Isolation and Characterization of Catalase-producing Bacteria from Soil
|
|
Han Kyung-Ah
Lee Jong-Il
|
|
Abstract
|
|
|
This study investigated the production of catalase from Bul-kyo soil bacteria through fermentation process. Isolation and selection of bacteria was performed through chemical and physiological analysis. Catalases were produced from bacteria which belong to 3 different species (Bacillaceae bacterium BKBChE-1, Bacillus sp. BKBChE-2, Bacillus flexus BKBChE-3) confirmed by using 16S rDNA sequence method. The catalases were found to be stable in the temperature range of 30¡É-60¡É for BKBChE-1, BKBChE-2 and BKBChE-3 and also in the pH range of 9.0-12.0 for BKBChE-1 and BKBChE-3. Long-term stability of the catalases was about 20 days at 4¡É. However, BKBChE-2 has kept its activity over 30 days at 4¡É.
|
|
KEYWORD
|
|
Catalase, Hydrogen peroxide, Bacillus sp., Identification, 16S rDNA
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|