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KMID : 0380020110260030223
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Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 3 p.223 ~ p.228
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Bioethanol Production Based on Crude Glycerol Using Enterobacter aerogenes
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Jung Hong-Sub
Seong Pil-Je
Go A-Ra
Lee Sang-Jun
Kim Seung-Wook
Han Sung-Ok
Cho Jae-Hoon
Cho Dae-Haeng
Kim Yong-Hwan
Park Chul-Hwan
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Abstract
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The effects of pH, glycerol concentration and salt on cell growth and ethanol production using Enterobacter aerogenes KCTC 2190 were evaluated in the anaerobic culture condition. In condition of initial pH 5, cell growth and ethanol production were highest. An initial concentration of 10 g/L of pure glycerol gave the highest cell growth and ethanol production. However, in case of over 15 g/L of pure glycerol, they decreased. The cell growth and ethanol production decreased with the increase of salt concentration. When 10 g/L of crude glycerol was used as the carbon source, the cell growth and ethanol production were 1.32 OD600 and 3.95 g/L, respectively, which were about 94.4% and 88.5% compared to those of pure glycerol. These result indicates that the crude glycerol produced in the biodiesel manufacturing process maybe useful as a potential carbon source for ethanol production form Enterobacter aerogenes KCTC 2190.
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KEYWORD
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Enterobacter aerogenes, ethanol, glycerol, crude glycerol
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