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KMID : 0380020190340040366
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 4 p.366 ~ p.376
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Understanding of Organic-Inorganic Composite Formation in Living Organisms by Experiments of Silica Synthesis Catalyzed by Organic Molecules
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Ki Mi-Ran
Min Ki-Ha
Pack Seung-Pil
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Abstract
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The discovery of the principle molecules involved in biosilicification both in diatoms and sponges brings out a new paradigm for silica synthesis at environmentally benign conditions. Here we introduce the biosynthetic method of silica using organic molecules for the undergraduate students. First, the biosilica synthesized was assessed through both a colorimetric method for quantification of silica and structural analysis by SEM image observation. Next, facile immobilization of an enzyme was demonstrated. Specifically, bovine carbonic anhydrase II (bCAII) was efficiently immobilized within silica matrix by simply adding itself to the reaction mixture containing polyamines and tetramethyl orthosilicate in phosphate buffer, obtaining 97% immobilization to initial amount of bCAII. The immobilized bCAII retained 40% of initial activity at 90℃ whereas free form did less than 10% of initial activity at 70℃ and the total yield from the 5 times reuses of immobilized bCAII was twice that of free bCAII. This experimental content that is hard to find in general biology or biochemistry lab could contribute to students' interest in knowledge expansion and application of new materials.
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KEYWORD
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biosilica, silica forming organic molecules, sol-gel reaction, molybdenum blue colormetric method, immobilization of enzyme
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