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KMID : 0380020200350020178
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Korean Journal of Biotechnology and Bioengineering
2020 Volume.35 No. 2 p.178 ~ p.182
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Enhanced Production of Poly(3-hydroxybutyrate) from Wood Hydrolysate by Recombinant Escherichia coli Expressing Response Regulator dr1558
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Gong Ryu-Gyoung
Choi Jun-Ho
Lee Jae-Won
Kim Tae-Wan
Choi Jong-Il
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Abstract
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In this study, wood hydrolysate was added in MR medium for the economical production of poly (3-hydroxybutyrate) by recombinant Escherichia coli. When response regulator dr1558 from Deinococcus radiodurans was overexpressed along with PHB biosynthesis genes, recombinant E. coli XB (pKM212_CnCAB+pRadGro_dr1558) had higher cell concentration and PHB content compared to control strain E. coli XB (pKM212_CnCAB+ pRadGro). From the transcriptional analysis by quantitative real time PCR, some genes involved in TCA cycle and PHB biosynthesis were overexpressed in E. coli XB (pKM212_CnCAB+pRadGro_dr1558). This results will be useful for production of biochemicals from cheap hydrolysate by fermentation process.
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KEYWORD
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wood hydrolysate, poly(3-hydroxybutyrate), response regulator dr1558, Escherichia coli
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