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KMID : 0380020200350040337
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Korean Journal of Biotechnology and Bioengineering
2020 Volume.35 No. 4 p.337 ~ p.341
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Production of 3-Hydroxypropionic Acid by Engineered Kluyveromyces marxianus
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Park Jae-Bum
Kang Kyoung-Gon
Kwon Deok-Ho
Ha Suk-Jin
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Abstract
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Thermotolerant yeast, Kluyveromyces marxianus was applied for the production of 3-hydroxypropionic acid (3- HP), which is mainly used as a raw material for biodegradable polymers. The MCR (malonyl-CoA reductase) gene originated from the Chloroflexus aurantiacus strain was introduced into the engineered K. marxianus 17555-JBP2 strain showing improved xylose utilization in the previous study to develop the engineered K. marxianus JBP2_MCR strain. At 40°C, K. marxianus JBP2_MCR strain produced 0.14±0.00 g/L of 3-HP using xylose as a sole carbon source. As a result of measuring the enzymatic activity of malonyl-CoA reductase, the engineered K. marxianus JBP2_MCR strain exhibited 4.71 times higher MCR enzymatic activity than the parent strain. When 1.28 g/L malonyl-CoA was added into media, 1.58±0.18 g/L of 3-HP was produced by K. marxianus JBP2_MCR strain for 120 h.
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KEYWORD
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Kluyveromyces marxianus, 3-Hyroxypropionate, malonyl-CoA, malonyl-CoA reductase
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