|
KMID : 0380219930260040328
|
|
Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 4 p.328 ~ p.331
|
|
|
Activation of c-src Protein Tyrosine Kinase by Caffeine in NIH 3T3 Cells Arrested in Early Sphase
|
|
Hee-Youn Hong
Jung-Kap Choi and Gyurng-Soo Yoo
|
|
|
Abstract
|
|
|
|
When NIH 3T3-derived c-src overexpresser cells synchronized in early S phase were exposed to caffeine, mitosis specific events such as the induction of round cell morphology and the activation of c-src protein tyrosine kinase were induced, and then disappeared upon further incubation. These mitosis specific events occurred even in the presence of cycloheximide or actinomycin D. Within 1 h of exposure to caffeine, c-src protein tyrosine kinase activity rose 2-3 fold compared with untreated controls, but returned to the control level upon further incubation. In contrast, when cells were treated by sod. vanadate, a protein tyrosine phosphatase inhibitor with caffeine simultaneously, no increase in c-src kinase activity was observed. These results suggest that caffeine sensitive tyrosine 527 phosphatase or kinase controls the activation of pp60c-src kinase.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|