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KMID : 0380219950280050437
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Journal of Biochemistry and Molecular Biology
1995 Volume.28 No. 5 p.437 ~ p.442
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Efficacy Tests of Recombinant Human Growth Hormone Produced from Saccharomyces cerevisiae
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Park Soon-Jae
Kim Nam-Joong
Kwon Soon-Chang
Lee Seung-Joo
Cho Joong-Myung
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Abstract
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The potency of yeast-derived methionyl-free human growth hormone (rhGH), which was obtained by removal of the N-terminal Met from methionyl-hGH, was estimated by in vitro and in vivo assays. In radio-receptor assay where the binding affinity of growth hormone to the receptor was estimated, the recombinant hGH showed 2.9 international units (IU) per mg of specific activity. In contrast, pitUitary-derived human growth hormone had a slightly lower receptor binding activity (2.5 IU/mg) compared with recombinant growth hormone. For the in vivo assay, efficacy of rhGH was tested by use of hypophysectomized rats, in which pituitary organs were surgically removed, resulting in the termination of growth hormone secretion. The weight-increase in rats by the injection of rhGH was almost identical to the result obtained by the injection of the same amount of pituitary-derived (international standard) hGH. A comparision of the secondary structures of rhGH and rMet-hGH by circular dichroism spectrophotometer demonstrated that the removal of the methionyl residue from rMet-hGH did not exert any effect on the structure of the growth hormone. In conclusion, methionyl-free human growth hormone produced from yeast was highly potent in biological activity and maintained a legitimate three dimensional structure.
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KEYWORD
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CD structure, human growth hormone, hypophysectomized rat, radio receptor assay, weightgain assay
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