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KMID : 0380420010250030151
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Journal of Prventive Veterinary Medicine
2001 Volume.25 No. 3 p.151 ~ p.163
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Serotyping and Molecular Typing of Salmonella Isolated from Herons and Environmental Samples in Geo-Je Island
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우용구/Woo, Yong Ku
박진영/이영주/권용국/모인필/Park, Jin Young/Lee, Young Ju/Gwun, Yong Gug/Mo, In Pil
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Abstract
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Since 1997, three large scale unknown death had consecutively appeared in the wild heron flocks in Geo-Je island until 1999. Until now, there has been only a little information on the Salmonella spp. originated from the wild animals in our peninsular. Hence, the objective of this study was to obtain the epidemiological characteristics related to Salmonella strains cultured from wild herons and environmental samples in Geo-Je island using the serotyping, antibiograms, plasmid profiles, and random amplified polymorphic of DNA (RAPD) profiles. A total of fifty-five Salmonella strains were isolated from liver and cecal contents of wild herons captured in Geo-Je island on August in 2000. Among the wild heron species tested, Ardea Cinerea jouyi (Clark) was shown the most high Salmonella isolation frequency (38.5%) and Egretta alba modesta (Gray) was followed as 15.4% (2/13), but any Salmonella could not isolate from the other species. Among the five serotypes identified, S. typhimurium (70.3%) was the most predominant serotype in the wild heron flocks and S. hadar (21.3%), S. wippra (4.3%), S. california (2.2%) and S. chincol (2.2%) were followed in order. S. wippra and S. chincol were reported as the first time among the domestic avian Salmonella serotypes since 1994. Three serotypes including S. typhimurium, S. hadar and S. chincol were commonly cultured from the internal organs of herons, whereas S. wippra was cultured only from the environmental sample, especially in the earth sample. In antimicrobial drug susceptibility test, all Salmonella strains from internal organs of heron were susceptible to all drugs tested, whereas strains from environmental samples were shown the resistance to carbenicillin, doxycyclin, tetracycline and oxytetracycline, respectively. Antibiogram pattern alone could discriminate into two types depending on their isolated origins. The serotype specific plasmid (60 MDa) was found in most of ST isolates (65%) and all strains had also the invA gene (284 bp) by PCR. These two genetic materials (SSP and invA gene) were known as a kind of virulence factors of pathogenic ST strains. The genetic relatedness was also compared between the ST strains from different sources using the random amplified polymorphism of DNA (RAPD) method. As a results, the present method was found as a rapid, efficient and useful tools to differentiate the same Salmonella serotype without specific difficulties in manipulation.
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