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KMID : 0380420010250040233
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Journal of Prventive Veterinary Medicine
2001 Volume.25 No. 4 p.233 ~ p.241
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Studies on the Proportions of Lymphocyte Subpopulations using Set of Monoclonal Antibodies Specific to Bovine Leukocyte Different Molecules in Mastitis-Resistant Cows and Mastitis-Susceptible Cows
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주이석/Joo, Yi Seok
문진산/김종택/서국현/위성환/남향미/박용호/Moon, Jin San/Kim, Jong Taek/Seo, Kook Hyun/Wee, Sung Hwan/Nam, Hyang Mi/Park, Yong Ho
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Abstract
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To investigate the disease defense system on mastitis of the dairy cows, proportions of lymphocyte subpopulations were examined using a set of MAbs specific to bovine leukocyte different molecules and flowcytometry in mastitis-resistant cows group and mastitis-susceptible cows group. The proportion of lymphocyte subpopulations of leucocyte seperated from milk and blood of these two groups were compared and analyzed. The results were as follows. Mean MHC-class Ⅱ DP-like, DQ, DR cells percentage of milk were 78.5%, 59.8%, and 68.0% in mastitis-resistant group, 31.2%, 31.2%, and 21.6 % in mastitis-susceptible group, respectively. There were significant differences between the two groups (p<0.05). The percentages of BoCD2+ and BoCD4+ T cells of milk and blood in mastitis-resistant group were both higher than those of mastitis-susceptible group. Especially in milk, the percentages of BoCD2 and BoCD4T cells were higher in resistant group cows (38.2%, 27.9%) than those of susceptible group cows (13.0%, 7.7%) (p<0.05). Mean B lymphocyte percentage of milk was 31.6% in resistant group and 15.7 % in susceptible group. The percentages of N lymphocyte of milk and blood were 10.1%, 6.0% in resistant group and 3.4%, 2.3% in susceptible group, respectively (p<0.05). Mean ratio of the BoCD4+/BoCD8+ T cells of milk and blood in resistant group cows were far higher than those of susceptible group cows. In milk and blood, it was 3.2, 2.5 in resistant group cows, and 0.42, 0.15 in susceptible group cows, respectively.
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