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KMID : 0380420020260010055
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Journal of Prventive Veterinary Medicine
2002 Volume.26 No. 1 p.55 ~ p.63
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Experimental Infection of Chickens with Brucella abortus Biotype 1
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Abstract
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Brucellosis, is one of the major zoonoses in Korea, and has been recognized as an agent of reproductive failure in dairy cattle, thereby causing significant economic losses through calf loss and in costs for regulatory and eradication programs. We hypothesized that avian species may serve as one of significant reservoir for mammalian brucellosis and that they remain largely undetected and could be used as experimental models for B. abortus Biotype 1 infection. In order to test the hypothesis, B. abortus biotype 1 was inocluated into domestic fowl and examined the bird culturally and immunologically using standard laboratory methods for diagnosis of brucellosis. Serial serum samples were collected and evaluated antibody titers by the plate agglutination test (PAT) and rose bengal test (RBT) using B.abortus 1119-3 antigen. Daily examination of fecal and necropsy samples revealed that B. abortus organisms persisted in the gastrointestinal tract, spleen, liver and ovaries for up to 35 days post-infection. The inf ection was successfully induced in all of inoculated chickens and none of the birds developed any clinical evidence of disease over 35 days of observation except febricula (40.8℃∼42.2℃) on 2 days post-infection and reducing growth of ovarian follicles. Seroconversion was achieved from all of inoculated birds. The earliest positive serological test was 2 days post-infection followed by RBT(1:100). The birds in control group showed for no evidence of infection. The study shows chicken can be experimental animal for model and avian brucellosis may be potential reservoir for brucellosis of public health importance.
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KEYWORD
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