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KMID : 0380420150390010023
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Journal of Prventive Veterinary Medicine
2015 Volume.39 No. 1 p.23 ~ p.26
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Genetic relationship of Campylobacter jejuni isolates from different sources by PFGE and flaA typing
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Ho Hung-Wui
Dong Hee-Jin
Kim Woo-Hyun
Bang Ki-Man
An Jae-Uk
Kim Chun-Hyung
Seo Kun-Ho
Hahn Tae-Wook
Ku Bok-Kyung
Cho Seong-Beom
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Abstract
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Thirty-one Campylobacter jejuni isolates (22 from various local sources, 9 from imported chicken meats) weresubtyped with PFGE and flaA typing to investigate their genetic relatedness. Based on a 90% similarity criterion, 23 and 21genotypic patterns were formed by PFGE and flaA typing, respectively. The discriminatory indices for PFGE, flaA typing, anda composite analysis of PFGE and flaA typing were 0.9785, 0.9527, and 0.9871, respectively. Similar patterns in compositeanalysis were observed between sources (cattle and chicken, and cattle and human), indicating that reservoir animals mayhave been the source of human campylobacteriosis. Therefore, strict hygiene measures from farm to table should beimplemented to prevent diseases due to C. jejuni in humans.
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KEYWORD
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Campylobacter jejuni, chicken, PFGE, flaAtyping
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