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KMID : 0380420160400040157
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Journal of Prventive Veterinary Medicine
2016 Volume.40 No. 4 p.157 ~ p.165
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Spheroid formation from equine mesenchymal stem cells
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Park Mi-Jeong
Lee Jienny
Sohn Yong-Woo
Kim Ha-Ki
Byeon Jeong-Su
Gu Na-Yeon
Cho In-Soo
Cha Sang-Ho
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Abstract
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Mesenchymal stem cells (MSCs) are primary candidates for cell therapy and tissue engineering applications. A two-dimensional (2D) culture system is typically used for cell growth, but that method affects the characteristics of stem cells. The physiological cell environment connects cells not only to each other, but also to the extracellular matrix providing mechanical support, exposing the entire cell surface, and opening signaling pathways. The hanging drop method is the most widely used 3D culture method for spheroid formation. In this study, we investigated the relationship between spheroid size and changes in gene expression to determine the optimum spheroid size for use in tissue engineering. The expression levels of stemness factors such as NANOG, OCT4, and SOX2, angiogenic factors such as VEGF and IL-8, and osteogenic factors such as COX2 and TGF-β1 increased with spheroid size in the respective spheroid formation groups unlike the responses in their monolayer groups. Therefore, our results indicate that spheroid formation through the hanging drop method can increase the efficiency of MSCs-based tissue engineering over that obtained via traditional 2D cell culture systems.
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KEYWORD
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Equine adipose tissue, mesenchymal stem cells, angiogenesis, osteogenesis
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