|
KMID : 0380420180420040157
|
|
Journal of Prventive Veterinary Medicine
2018 Volume.42 No. 4 p.157 ~ p.162
|
|
|
Immunization with Brucella abortus recombinant proteins protects BALB/c mice from Brucella abortus 544 infection
|
|
Arayan Lauren Togonon
Huy Tran Xuan Ngoc
Reyes Alisha Wehdnesday Bernardo
Hop Huynh Tan
Son Vu Hai
Min Won-Gi
Lee Hu-Jang
Kim Suk
|
|
|
Abstract
|
|
|
|
This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response.
|
|
|
KEYWORD
|
|
|
B. abortus, recombinant proteins, vaccine, cytokine, protection
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|