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KMID : 0381120060280040433
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Genes and Genomics
2006 Volume.28 No. 4 p.433 ~ p.441
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Cloning and Subcellular Localization of a Serpin containing Nuclear Export Signal from the Korean malaria vector, Anopheles sinensis
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Noh Mi-Young
Jo Yong-Hun
Oh Seung-Han
Kim Dong-Hyun
Park Hee-Jung
Kim Ik-Soo
Carolina Barillas-Mury
Kim Heung-Chul
Lee Won-Ja
Lee In-Hee
Seo Sook-Jae
Kang Se-Won
Lee Yong-Seok
Kho Weon-Gyu
Kang Sang-Sun
Han Yeon-Soo
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Abstract
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Anopheles sinensis is known to play a critical role in malaria transmission and re-emergence in the areas near the Demilitarized Zone (DMZ) of Korea. However, no study on Plasmodium-midgut interactions using A. sinensis has been reported. Here, we describe the cloning and dynamic subcellular localization of the orthologue of Anopheles gambiae (AgSRPN10), isoform RCM, from Anopheles sinensis (AnsiSRPN10). AnsiSRPN10 mRNA is expressed in embryoes, and is almost undetectable in 4th instar larvae. It increases transiently in pupae and is most abundant in adult females. Expression is higher in the abdomen and the midgut compared to the thorax and ovary. It is induced in response to laminarin and Actinomycin-D. AnsiSRPN10 protein does not contain a consensus nuclear localization signal (NLS), but has a putative nuclear export signal (NES) and small ubiquitin-like modifier (SUMO) modification site. It is present mainly in the nucleus of healthy midgut cells, but translocates from the nucleus to the cytosol in Plasmodium-invaded cells. AnsiSRPN10 expression increases as midgut cells undergo apoptosis, indicating that the epithelial responses to P. berghei invasion are conserved across different anopheline species. AnsiSRPN10 is a useful marker of Plasmodium-induced apoptosis in midgut. To our knowledge, this is the first report on A. sinensis innate immunity in the context of Time Bomb model.
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KEYWORD
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Anopheles sinensis, Plasmodium berghei, Serpin, Midgut, Apoptosis, Time Bomb model
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