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KMID : 0381120080300060541
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Genes and Genomics
2008 Volume.30 No. 6 p.541 ~ p.548
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Human Serine Hydroxymethyltransferase 2 (SHMT2) Iinteracts with hnRNP D
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Choi Mie-Young
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Abstract
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Heterogeneous nuclear ribonucleoprotein D (hnRNP D) is one of the principal pre-mRNA binding proteins present in human cells. It is a ubiquitously expressed, nuclear protein. The protein contains two RNP-consensus RNA-binding domains and a glycine-rich C-terminal domain. It has been known to be associated with the regulation of mRNA stability by binding to AU-rich elements (ARE) present in the 3’ UTR of several mRNAs. In order to identify cellular proteins that bind to hnRNP D, this research conducted yeast two-hybrid screening using a HeLa cDNA library. It then discovered that one of the cDNA clones carried a partial human serine hydroxymethyltransferase 2 (mitochondrial) (SHMT2) cDNA (GenBank accession number NM_005412). This paper shows for the first time that human serine hydroxymethyltransferase 2 binds specifically
to hnRNP D, both in yeast two-hybrid system and in vitro pull-down assay.
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KEYWORD
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heterogeneous nuclear ribonucleoprotein D (hnRNP D/AUF1), RNP-concensus RNA-binding domain, yeast two-hybrid screening system, human serine hydroxymethyltransferase 2 (SHMT2)
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