KMID : 0381120110330010009
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Genes and Genomics 2011 Volume.33 No. 1 p.9 ~ p.15
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Applications of cell-based phage display panning to proteomic analysis
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Kim Kristine M.
Kang Min-Jeong Yi Eu-Gene C.
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Abstract
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In the post-genomic era, proteomics together with genomic tools have led to powerful new strategies in basic and clinical research. These combined ¡°omics¡± technologies are being integrated into the drug target discovery process. Unlike the genome, the proteome is a highly dynamic entity that requires techniques capable of analyzing on selected populations of proteins in specific biological conditions that reflect the proteins¡¯ functional characteristics. Antibodies have become one of the most important reagents for the analysis of selected populations of proteins, and the application of phage-display antibody libraries to high-throughput antibody generation against large numbers of various antigens provides a tool for proteome-wide protein expression analysis. In this review, we will discuss the utility of phage-display antibodies in proteomics applications, specifically for the discovery of novel disease markers and therapeutic targets.
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KEYWORD
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Proteomics, Phage display, Antibody, Mass spectrometry, Herapeutics
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