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KMID : 0381120120340060647
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Genes and Genomics
2012 Volume.34 No. 6 p.647 ~ p.652
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Characterization of the hamster genomic fragment cloned by TAR cloning technology with interspecific sequence information
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Lee Sang-Yeop
Yoon Young-Ho
Kim Si-Hoon
Lee Se-Ra
Chu Jeong-Min
Kim Seung-Il
Kang Tae-Hong
Chung Jin-Woong
Larionov Vladimir
Leem Sun-Hee
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Abstract
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CHO (Chinese Hamster ovary) cells are widely used for biotechnology and biomedical purposes, and now the EST library database of CHO cells is built. Based on this, the construction of the hamster genome library is under exertion. Though the transformation-associated recombination (TAR) cloning method is accounted as an innovative cloning technology without the construction of the genome library in human and mouse, there has been no trial to isolate the genomic fragment from hamster genome by TAR cloning. In this study, approximately 31 kb of hamster genomic fragment was isolated from the normal human/hamster mono-chromosomal somatic cell line (UV5HL9-5B) using universal hooks of rodent repeats sequence of B1 and B2 by TAR cloning. This fragment was analyzed by bioinformatics tools related to the genome alignment for the similarity analysis among rodent and primate, and was classified into rodents by phylogenetic analysis. One putative gene was found in this region which has homology with the human c14orf4 gene. A zinc finger protein domain was found in the translated hamster ORF. Therefore, we suggest that TAR cloning technique can be applied in CHO cells using mouse genomic information, and it can lead to the establishment of the hamster genome database.
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KEYWORD
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Hamster genome, TAR cloning, c14orf4, B1-B2 repeats
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