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KMID : 0381120180400111237
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Genes and Genomics
2018 Volume.40 No. 11 p.1237 ~ p.1248
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Effect of 1-aminocyclopropane-1-carboxylic acid (ACC)-induced ethylene on cellulose synthase A (CesA) genes in flax (Linum usitatissimum L. ‘Nike’) seedlings
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Lim Han-Sol
Paek Seung-Ho
Oh Seung-Eun
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Abstract
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Introduction: Cellulose microfibril is a major cell wall polymer that plays an important role in the growth and development of plants. The gene cellulose synthase A (CesA), encoding cellulose synthases, is involved in the synthesis of cellulose microfibrils. However, the regulatory mechanism of CesA gene expression is not well understood, especially during the early developmental stages.
Objective: To identify factor(s) that regulate the expression of CesA genes and ultimately control seedling growth and development.
Methods: The presence of cis-elements in the promoter region of the eight CesA genes identified in flax (Linum usitatissimum L. ‘Nike’) seedlings was verified, and three kinds of ethylene-responsive cis-elements were identified in the promoters. Therefore, the effect of ethylene on the expression of four selected CesA genes classified into Clades 1 and 6 after treatment with 10?4 and 10?3 M 1-aminocyclopropane-1-carboxylic acid (ACC) was examined in the hypocotyl of 4?6-day-old flax seedlings.
Results: ACC-induced ethylene either up- or down-regulated the expression of the CesA genes depending on the clade to which these genes belonged, age of seedlings, part of the hypocotyl, and concentration of ACC.
Conclusion: Ethylene might be one of the factors regulating the expression of CesA genes in flax seedlings.
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KEYWORD
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CesA, Gene expression, Ethylene, 1-Aminocyclopropane-1-carboxylic acid, Flax, Seedlings
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