Conidio spores of Aspergillus niger (strain No. NRRL 330) cultured on potato dextrose agar media were studied by electron microscopy, using the thin sectioning techniques. Conidio spores to be sectioned were fixed by triple methods with KZCr2O7, Glutaraldehyde and OsO4. After dehydrated with alcohol, the specimens were embedded in metacrylate and epon resin media, and thinly sectioned, by Porter-Blum MT-2. After sectioned these specimens were. negative-stained with uranyl acetate and observed, by Hitachi HS-6 electron microscope.
The results of this experiment were summarized as follows.
1. The structures of spore wall system seem to be formed 4 layers; exosporium, basal layer, spore coat and unit cell membrane. The protuberance of spore surface that was looked like hair appears to be protrusived from the basal layer.
2. The, 3 layers of unit cell membrane was constituted outer layer membrane, inner Gayer membrane. and intermediate light layer.
3.The structures of intra cytoplasmic membrane appear as spiral form which was consisted. of 3 layers membrane system; outer membrane inner membrane, and intermediate layer which has pits.
4. The cement substance of spore coat. and cortex may be changed quantitatively by .physiological . state in cell.
5. In some cases we observed that the ribosome was transformed into poly ribosome group and the storage materials and the protein crystals were changed variously. It has been suggested that the morphological change of some cytoplasmic materials may be caused by some specialized function of the physiological stage.
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